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Antibacterial peptides have attracted a- great deal of attention due to their biological properties against a wide range of microorganisms
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Maryam Shokoohmand Dr. *   , Hossein Zolgharnein Dr. , Mohammad Ali Salari Ali Abadi Dr. , Mojtaba Alishahi Dr. , Reza Safari Dr. |
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Abstract: (93 Views) |
Introduction: The present study aimed to investigate antibacterial and properties of proteins produced by the enzyme hydrolysis of lantern fish (Benthosema petrotum) produced by alcalase and flavourzyme enzymes.
Materials & Methods: Membrane ultrafiltration method was used to isolate and purify antimicrobial peptides smaller than 3 kDa. Antibacterial activity of protein hydrolyzed with alkalase enzyme (BPHA) and flavourzyme enzyme (BPHF) against Aeromonas hydrophila, Streptococcus agalactiae, Sterptococcus iniae, Yersinia ruckeri (standard strains) was performed by disk diffusion and microdilution method.
Results: The results showed the appropriate efficiency of enzymes used to isolate antimicrobial peptides smaller than 3 kDa from lantern fish. Also, the lowest amount of MIC, MBC of hydrolyzed protein by flavourzyme against bacteria S, iniae was 16 and 32 μg / ml. The diameter of the growth inhibition zone of peptides isolated with alkalase enzyme (BPHA) and flavourzyme enzyme (BPHF) against Y. ruckeri and S. iniae was competitive with tetracycline antibiotic.
Conclusion: In general, it can be concluded that bioactive peptides derived from lantern fish have relative antibacterial effects against fish bacterial agents, although this effect depends on the type of enzyme used and the bacterial species. Therefore, further research on the possibility of using these antibacterial agents for fish bacterial pathogens is recommended. |
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| Keywords: Aeromunas hydrophyila, Antibacterial properties, Bioactive peptides, Lantern fish, Streptococcus agalactiae, Sterptococcu iniae, Yersini ruckeri |
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Full-Text [PDF 741 kb]
(32 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2026/08/12 | Accepted: 2025/04/30 | Published: 2025/04/30
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