fa بررسی اثرات ضدقارچی فرم نانو و خام ژل رویال زنبورعسل بر قارچ آسپرژیلوس فلاووس (Aspergillus flavus) تخصصي Special مروری Review <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مقدمه:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> به</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">دلیل</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مقاومت</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">روزافزون</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">باکتری</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">ها</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">و قارچ</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">های</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">بیماری</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">زا</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">به</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">آنتی</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">بیوتیک</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">های</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">رایج، یافتن</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">عوامل ضدمیکروبی</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">طبیعی به</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">عنوان</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">داروهای</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">جایگزین</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">اهمیت دارد، از آن</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">جاکه قارچ آسپرژیلوس فلاووس یکی از قارچ</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">های مهم مولد تولید آفلاتوکسین می</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">باشد. در این تحقیق اثرات ضدقارچی ژل رویال زنبورعسل و مشتقات نانوکامپوزیت آن بر روی قارچ </span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Aspergillus flavus</span></span></span></i><i> </i><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مورد مطالعه قرار گرفت. </span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مواد و روش&shy;ها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> برای انجام این تحقیق نمونه ژل رویال از زنبورستان</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">های استان کرمان جمع</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">آوری</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">شده و در آزمایشگاه عصاره</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">گیری به</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">عمل آمد</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">.</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> سپس قارچ </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">&nbsp;</span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Aspergillus flavus</span></span></span></i><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> در محیط اختصاصی کشت داده شده و تحت تاثیر رقت</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">های مختلف عصاره</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">های ژل رویال، نانوکامپوزیت</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">های سپیولایت/ژل رویال و سپیولایت/ نیترات نقره/ ژل رویال قرار گرفت. در</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">نهایت </span></span></span>&nbsp;<span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">MIC</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">MFC</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> نمونه</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">ها توسط تست</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">دیسک دیفوژن</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">و</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">چاهک پلیت تعیین</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">گردید. اطلاعات به</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">دست آمده توسط نرم</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">افزار</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">SAS</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> مورد</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">تجزیه و</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">تحلیل</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">قرار گرفت. </span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">نتایج:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> نتایج این تحقیق نشان داد که اگرچه عصاره</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">های ژل رویال و ترکیبات نانوی آن در مقایسه با آنتی</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">بیوتیک مورد استفاده بر علیه قارچ آسپرژیلوس فلاووس داری خاصیت ضدقارچی کم</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">تری بودند (0/01</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">P<</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">). اما تاثیر معنی</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">داری بر میزان مهار و کشندگی قارچ آسپرژیلووس</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">فلاووس داشته</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">اند. به</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">طوری</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">که ژل رویال به</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">صورت بالک و نانوکامپوزیت در</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">غلظت </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">mg/ml</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">۴۰۰ دارای خاصیت مهار و کشندگی بر روی قارچ مورد نظر بودند. هم</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">چنین عصاره نانوکامپوزیت سپیولایت/نیترات نقره/ ژل رویال به</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">میزان ناچیز اما معنی</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">داری اثر ضدقارچی بیش</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">تری نسبت به عصاره خام داشت (0/01</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">P<</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">). </span></span></span></span></span></span></span></span><br> <b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">بحث و نتیجه</span></span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">گیری:</span></span></span></span></b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black"> بنابراین می</span></span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">توان دریافت که ژل رویال می</span></span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">تواند به</span></span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">عنوان یک ضدقارچ طبیعی به</span></span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">خصوص در فرم نانو مورد استفاده قرار گیرد</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">.</span></span></span></span></div> داروی کم خطر, اثرات مهارکنندگی, آسپرژیلوس فلاووس, زنبورعسل, نانو‌ذرات 107 114 http://journalaer.com/browse.php?a_code=A-10-1-13&slc_lang=fa&sid=1 Elham Rezvannejad الهام رضوان نژاد e.rezvannejad@kgut.ac.ir 1003194753284600543 1003194753284600543 Yes Maryam Fayazi مریم فیاضی 1003194753284600544 1003194753284600544 No Batool Sadeghi بتول صادقی 1003194753284600545 1003194753284600545 No